| Autor: |
Linnertz, Holger, Kost, Holger, Obsil, Tomas, Kotyk, Arnost, Amler, Evzen, Schoner, Wilhelm |
| Zdroj: |
FEBS Letters; December 1998, Vol. 441 Issue: 1 p103-105, 3p |
| Abstrakt: |
The high‐affinity E1ATP site of Na+/K+‐ATPase labeled with fluorescein 5′‐isothiocyanate and its E2ATP site labeled with erythrosin 5′‐isothiocyanate (ErITC), as was shown recently [Linnertz et al. (1998) J. Biol. Chem. 273, 28813–28821], reside on separate and adjacent catalytic α subunits. This paper provides evidence that specific labeling of the E2ATP binding site with ErITC resulted in a modification of the Cys549residue in the tryptic fragment with the sequence Val545‐Leu‐Gly‐Phe‐Cys549‐His550. Hence, Cys549is part of or close to the low‐affinity E2ATP binding site of Na+/K+‐ATPase. |
| Databáze: |
Supplemental Index |
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