| Autor: |
Cadena, Silvia M.S.C, Carnieri, Eva G.S, Echevarria, Aurea, de Oliveira, Maria Benigna M |
| Zdroj: |
FEBS Letters; November 1998, Vol. 440 Issue: 1-2 p46-50, 5p |
| Abstrakt: |
MI‐D (4‐phenyl‐5‐(4‐nitro‐cinnamoyl)‐1,3,4‐thiadiazolium‐2‐phenylamine chloride), a new mesoionic compound, depressed the phosphorylation efficiency of liver mitochondria as deduced from an accentuated decrease of the respiratory control coefficient and ADP/O ratio. Analysis of segments of the respiratory chain suggested that the MI‐D inhibition site is further on than complex I and between complexes II and III. The transmembrane electrical potential (Δψ) was collapsed dependent on MI‐D concentration. ATPase activity was dramatically increased by MI‐D in intact mitochondria, but inhibited in carbonylcyanide p‐trifluoromethoxyphenylhydrazone (FCCP)‐uncoupled mitochondria. These results suggest that MI‐D acts as an uncoupler agent, a property closely related to its structural characteristics. |
| Databáze: |
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