Phorbol ester markedly increases the sensitivity of intestinal epithelial cells to 1,25‐dihydroxyvitamin D3

Autor:	Armbrecht, H.James, Hodam, Tara L., Boltz, Monica A., Chen, Ming L.
Zdroj:	FEBS Letters; July 1993, Vol. 327 Issue: 1 p13-16, 4p
Abstrakt:	We have used a clonal intestinal epithelial cell line (IEC‐18) to study the mechanism of action of 1,25‐dihydroxyvitamin D3(1,25(OH)2D) in vitro. 1,25(OH)2D (10−7M) elevated by over 10‐fold the mRNA levels for the cytochrome P450 component (P450cc24) of the 1,25(OH)2D‐24‐hydroxylase. Increased P450cc24 mRNA levels were detectable at 6 h and peaked at 36 h. Below a concentration of 10−7M, 1,25(OH)2D had almost no effect. However, addition of phorbol ester for 2 h made the intestine responsive to 1,25(OH)2D concentrations as low as 10−9M.
Databáze:	Supplemental Index
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