| Autor: |
Helps, Nicholas R., Street, Alasdair J., Elledge, Stephen J., Cohen, Patricia T.W. |
| Zdroj: |
FEBS Letters; February 1994, Vol. 340 Issue: 1-2 p93-98, 6p |
| Abstrakt: |
The yeast two hybrid system has been employed to identify cDNAs encoding proteins which interact with the γ1, isoform of human protein phosphatase 1. Here we report the isolation of a cDNA encoding human protein phosphatase inhibitor 2. The deduced human sequence of 205 amino acids shows 92% identity to inhibitor 2 from rabbit. Human inhibitor 2 was expressed in E. coliand purified to homogeneity. The expressed human protein inhibited both native and bacterially expressed PP1, with the same Ki(1 nM) as inhibitor 2 purified from skeletal muscle. A gene or pseudogene for inhibitor 2 may be present near the major histocompatibility complex on chromosome 6. |
| Databáze: |
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