A novel plant glutathione peroxidase‐like protein provides tolerance to oxygen radicals generated by paraquat in Escherichia coli

Autor: Holland, Doron, Faltin, Zehava, Perl, Avi, Ben-Hayyim, Gozal, Eshdat, Yuval
Zdroj: FEBS Letters; January 1994, Vol. 337 Issue: 1 p52-55, 4p
Abstrakt: Citrus salt‐stress associated protein (Cit‐SAP) reveals significant sequence homology to mammalian glutathione peroxidase (GP). In an attempt to assign biological function to this protein, transformed E. colicells expressing Cit‐SAP were examined for their ability to tolerate free radicals formed by paraquat, an O−2radical forming agent. In the presence of paraquat, the survival rate of the transformed bacteria expressing Cit‐SAP was much higher as compared to the wild‐type bacteria. The results support the assumption that Cit‐SAP is a plant GP‐like protein which participate in the enzymatic system aimed at scavenging oxygen free‐radicals in plants.
Databáze: Supplemental Index