| Autor: |
Murray, Brendan E., Ohlendieck, Kay |
| Zdroj: |
FEBS Letters; June 1998, Vol. 429 Issue: 3 p317-322, 6p |
| Abstrakt: |
Linkage between the high‐capacity Ca2+‐binding protein calsequestrin and the ryanodine receptor is proposed to be essential for proper Ca2+‐release during skeletal muscle excitation‐contraction coupling. However, no direct biochemical evidence exists showing a connection between these high‐molecular‐mass complexes in native skeletal muscle membranes. Here, using immunoblot analysis of chemically crosslinked membrane vesicles enriched in triad junctions, we have demonstrated that a very close neighborhood relationship exists between calsequestrin and the ryanodine receptor in both main fiber types. Hence, the luminal Ca2+‐reservoir complex appears to be directly coupled to the membrane Ca2+‐release complex and oligomerization seems to be of functional importance. |
| Databáze: |
Supplemental Index |
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