| Autor: |
Kim, Jae Ho, Lee, Sang Do, Han, Jung Min, Lee, Taehoon G, Kim, Yong, Park, Jong Bae, Lambeth, J.David, Suh, Pann-Ghill, Ryu, Sung Ho |
| Zdroj: |
FEBS Letters; July 1998, Vol. 430 Issue: 3 p231-235, 5p |
| Abstrakt: |
Phospholipase D1 (PLD1) is known to be activated by ADP‐ribosylation factor 1 (ARF1). We report here that ARF1 co‐immunoprecipitates with PLD1 and that the ARF1‐dependent PLD activation is induced by the direct interaction between ARF1 and PLD1. We found that RalA, another member of the small GTP‐binding proteins, synergistically enhances the ARF1‐dependent PLD activity with an EC50of about 30 nM. Using in vitro binding assay, we show that ARF1 and RalA directly interact with different sites of PLD1. The results suggest that the independent interactions of RalA and ARF1 with PLD1 are responsible for the synergistic activation. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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