| Autor: |
Abrahamsson, Hakan, Berggren, Per-Olof, Rorsman, Patrik |
| Zdroj: |
FEBS Letters; October 1985, Vol. 190 Issue: 1 p21-24, 4p |
| Abstrakt: |
The effects of the hypoglycemic sulfonylureas tolbutamide and glibenclamide on free cytoplasmic Ca2+, [Ca2+]i, were compared with that of a depolarizing concentration of K+in dispered and cultured pancreatic β‐cells from ob/obmice. [Ca2+]iwas measured with the fluorescent Ca2+‐indicator quin2. The basal level corresponded to 150 nM and increased to 600 nM after exposure to 30.9 mM K+. The corresponding levels after stimulation with 1 μM glibenclamide and 100 μM tolbutamide were 390 and 270 nM respectively. K+depolarization increased [Ca2+]imore rapidly than either of the sulfonylureas. It is suggested that the increased [Ca2+]iobtained after stimulation by sulfonylureas is due to depolarization of the β‐cells with subsequent entry of Ca2+through voltage‐dependent channels. |
| Databáze: |
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