| Autor: |
Robberecht, Patrick, Waelbroeck, Magali, De Neef, Philippe, Tastenoy, Michèle, Gourlet, Philippe, Cogniaux, Jacqueline, Christophe, Jean |
| Zdroj: |
FEBS Letters; February 1988, Vol. 228 Issue: 2 p351-355, 5p |
| Abstrakt: |
A new type of VIP receptor was characterized in human SUP‐T1 lymphoblasts. The order of potency of unlabeled peptides in the presence of [125I]helodermin, was: helodermin(1–35)‐NH2= helodermin(1–27)‐NH2> helospectin > VIP = PHI > [D‐Ser2]VIP > [D‐Asp3]VIP > [D‐His1]VIP ⩾ [D‐Ala4]VIP 2̆ secretin = GRF. This specificity was distinct from that of all VIP receptors described so far in that: (i) the affinity for helodermin (Kd= 3 nM) was higher than that of VIP (Kd= 15 nM) and PHI (Kd= 20 nM); and (ii) position 4 played an important role in ligand binding. The labeled sites were likely to be functional receptors as adenylate cyclase in crude lymphoblastic membranes (200‐10 000 × gpellets) was stimulated by peptides, in the presence of GTP, with the following order of potency: helodermin(1–35)‐NH2> helo‐dermin(1–27)‐NH2> helospectin = VIP = PHI. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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