| Autor: |
Filipeanu, Catalin M., Nelemans, Adriaan, Veldman, Robert Jan, de Zeeuw, Dick, Kok, Jan Willem |
| Zdroj: |
FEBS Letters; May 2000, Vol. 474 Issue: 1 p107-110, 4p |
| Abstrakt: |
Regulation of capacitative Ca2+entry was studied in two different multidrug resistance (MDR) protein (MRP1) overexpressing cell lines, HT29coland GLC4/ADR. MRP1 overexpression was accompanied by a decreased response to thapsigargin. Moreover, inhibition of capacitative Ca2+entry by D,L‐threo‐1‐phenyl‐2‐decanoylamino‐3‐morpholino‐1‐propanol (PDMP) was abolished in MRP1 overexpressing cells. Both PDMP and the MRP1 inhibitor MK571 greatly reduced InsP3‐mediated 45Ca2+release from intracellular stores in HT29 cells. Again, these effects were virtually abolished in HT29colcells. Our results point to a modulatory role of MRP1 on intracellular calcium concentration ([Ca2+]i) homeostasis which may contribute to the MDR phenotype. |
| Databáze: |
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