| Autor: |
Nishimura, T., Bignon, C., Allouch, J., Czjzek, M., Darbon, H., Watanabe, T., Henrissat, B. |
| Zdroj: |
FEBS Letters; June 2001, Vol. 499 Issue: 1-2 p187-190, 4p |
| Abstrakt: |
The laminaripentaose‐producing β‐1,3‐glucanase of Streptomyces matensisis a member of the glycoside hydrolase family GH‐64. We have constructed and purified a recombinant hexahistidine‐tagged form of the enzyme for characterisation. The enzyme, which exists as a monomer in solution, hydrolyses β‐1,3‐glucan by a mechanism leading to overall inversion of the anomeric configuration. This is the first determination of the mechanism prevailing in glycoside hydrolase family GH‐64 and this is the first characterisation of an ‘inverting’ β‐1,3‐glucanase. |
| Databáze: |
Supplemental Index |
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