| Autor: |
Schmid, Volkmar H.R., Thomé, Peter, Rühle, Wolfgang, Paulsen, Harald, Kühlbrandt, Werner, Rogl, Hans |
| Zdroj: |
FEBS Letters; June 2001, Vol. 499 Issue: 1-2 p27-31, 5p |
| Abstrakt: |
Chlorophyll (Chl) molecules attached to plant light‐harvesting complexes (LHC) differ in their spectral behavior. While most Chl aand Chl bmolecules give rise to absorption bands between 645 nm and 670 nm, some special Chls absorb at wavelengths longer than 700 nm. Among the Chl a/b‐antennae of higher plants these are found exclusively in LHC I. In order to assign this special spectral property to one chlorophyll species we reconstituted LHC of both photosystem I (Lhca4) and photosystem II (Lhcb1) with carotenoids and only Chl aor Chl band analyzed the effect on pigment binding, absorption and fluorescence properties. In both LHCs the Chl‐binding sites of the omitted Chl species were occupied by the other species resulting in a constant total number of Chls in these complexes. 77‐K spectroscopic measurements demonstrated that omission of Chl bin refolded Lhca4 resulted in a loss of long‐wavelength absorption and 730‐nm fluorescence emission. In Lhcb1 with only Chl blong‐wavelength emission was preserved. These results clearly demonstrate the involvement of Chl bin establishing long‐wavelength properties. |
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