| Autor: |
Monteiro, Rose A., de Souza, Emanuel M., Wassem, Roseli, Yates, M.Geoffrey, Pedrosa, Fabio O., Chubatsu, Leda S. |
| Zdroj: |
FEBS Letters; November 2001, Vol. 508 Issue: 1 p1-4, 4p |
| Abstrakt: |
Herbaspirillum seropedicaeis an endophytic diazotroph, which colonizes sugar cane, wheat, rice and maize. The activity of NifA, a transcriptional activator of nifgenes in H. seropedicae, is controlled by ammonium ions through a mechanism involving its N‐terminal domain. Here we show that this domain interacts specifically in vitro with the N‐truncated NifA protein, as revealed by protection against proteolysis, and this interaction caused an inhibitory effect on both the ATPase and DNA‐binding activities of the N‐truncated NifA protein. We suggest that the N‐terminal domain inhibits NifA‐dependent transcriptional activation by an inter‐domain cross‐talk between the catalytic domain of the NifA protein and its regulatory N‐terminal domain in response to fixed nitrogen. |
| Databáze: |
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