| Abstrakt: |
The manganese peroxidase produced by Phanerochaete chrysosporium, which catalyzes the oxidation of Mn2+to Mn3+, is easily inactivated by the hydrogen peroxide (H2O2) presented in the reaction. We attempted to increase H2O2resistance by the conformational stabilization around the H2O2‐binding pocket. Based on its structural model, engineering of oxidizable Met273 located near the pocket to a non‐oxidizable Leu showed a great improvement. Furthermore, after treatment at 1 mM H2O2where the wild‐type is completely inactivated, full activity can be retained by engineering the Asn81, which might have conformational changes due to the environment of the pocket, to a non‐bulky and non‐oxidizable Ser. |
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