| Autor: |
Ziman, Michael, Chuang, John S., Tsung, Michael, Hamamoto, Susan, Schekman, Randy |
| Zdroj: |
Molecular Biology of the Cell; June 1998, Vol. 9 Issue: 6 p1565-1576, 12p |
| Abstrakt: |
Chitin synthase III (CSIII), an enzyme required to form a chitin ring in the nascent division septum of Saccharomyces cerevisiae, may be transported to the cell surface in a regulated manner. Chs3p, the catalytic subunit of CSIII, requires the product of CHS6to be transported to or activated at the cell surface. We find that chs6Δstrains have morphological abnormalities similar to those of chs3mutants. Subcellular fractionation and indirect immunofluorescence indicate that Chs3p distribution is altered in chs6mutant cells. Order-of-function experiments usingend4–1(endocytosis-defective) and chs6mutants indicate that Chs6p is required for anterograde transport of Chs3p from an internal endosome-like membrane compartment, the chitosome, to the plasma membrane. As a result, chs6strains accumulate Chs3p in chitosomes. Chs1p, a distinct chitin synthase that acts during or after cell separation, is transported normally in chs6mutants, suggesting that Chs1p and Chs3p are independently packaged during protein transport through the late secretory pathway. |
| Databáze: |
Supplemental Index |
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