| Autor: |
Mullen, Gregory P., Mathews, Eleanor A., Saxena, Paurush, Fields, Stephen D., McManus, John R., Moulder, Gary, Barstead, Robert J., Quick, Michael W., Rand, James B. |
| Zdroj: |
Molecular Biology of the Cell; July 2006, Vol. 17 Issue: 7 p3021-3030, 10p |
| Abstrakt: |
Sodium-dependent neurotransmitter transporters participate in the clearance and/or recycling of neurotransmitters from synaptic clefts. The snf-11gene in Caenorhabditis elegansencodes a protein of high similarity to mammalian GABA transporters (GATs). We show here that snf-11encodes a functional GABA transporter; SNF-11–mediated GABA transport is Na+and Cl−dependent, has an EC50value of 168 μM, and is blocked by the GAT1 inhibitor SKF89976A. The SNF-11 protein is expressed in seven GABAergic neurons, several additional neurons in the head and retrovesicular ganglion, and three groups of muscle cells. Therefore, all GABAergic synapses are associated with either presynaptic or postsynaptic (or both) expression of SNF-11. Although a snf-11null mutation has no obvious effects on GABAergic behaviors, it leads to resistance to inhibitors of acetylcholinesterase. In vivo, a snf-11null mutation blocks GABA uptake in at least a subset of GABAergic cells; in a cell culture system, all GABA uptake is abolished by the snf-11mutation. We conclude that GABA transport activity is not essential for normal GABAergic function in C. elegansand that the localization of SNF-11 is consistent with a GABA clearance function rather than recycling. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
