| Autor: |
Fowler, Thomas J., DeSimone, Susan M., Mitton, Michael F., Kurjan, Janet, Raper, Carlene A. |
| Zdroj: |
Molecular Biology of the Cell; August 1999, Vol. 10 Issue: 8 p2559-2572, 14p |
| Abstrakt: |
The mushroom-producing fungus Schizophyllum communehas thousands of mating types defined, in part, by numerous lipopeptide pheromones and their G protein-linked receptors. Compatible combinations of pheromones and receptors encoded by different mating types regulate a pathway of sexual development leading to mushroom formation and meiosis. A complex set of pheromone–receptor interactions maximizes the likelihood of outbreeding; for example, a single pheromone can activate more than one receptor and a single receptor can be activated by more than one pheromone. The current study demonstrates that the sex pheromones and receptors of Schizophyllum, when expressed inSaccharomyces cerevisiae, can substitute for endogenous pheromone and receptor and induce the yeast pheromone response pathway through the yeast G protein. Secretion of activeSchizophyllumpheromone requires some, but not all, of the biosynthetic machinery used by the yeast lipopeptide pheromonea-factor. The specificity of interaction among pheromone–receptor pairs in Schizophyllumwas reproduced in yeast, thus providing a powerful system for exploring molecular aspects of pheromone–receptor interactions for a class of seven-transmembrane-domain receptors common to a wide range of organisms. |
| Databáze: |
Supplemental Index |
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