| Autor: |
Wright, M., Dawson, J., Dunder, E., Suttie, J., Reed, J., Kramer, C., Chang, Y., Novitzky, R., Wang, H., Artim-Moore, L. |
| Zdroj: |
Plant Cell Reports; July 2001, Vol. 20 Issue: 5 p429-436, 8p |
| Abstrakt: |
A selectable marker system for plant transformation that does not require the use of antibiotics or herbicides was developed. The selectable marker consists of themanAgene fromEscherichia coliunder the control of a plant promoter that encodes for phosphomannose isomerase,pmi. Only transgenic plants were able to metabolize the selection agent, mannose, into a usable source of carbon, fructose. Transgenic plants were produced efficiently after delivery by Biolistics™ of thepmigene into maize and wheat tissues, with mean transformation frequencies of 45% for maize and 20% for wheat. Adjustment of the sucrose and mannose levels in the selection medium essentially eliminated escapes. Transgenic events can be identified as early as 2 months for wheat and 4 months for maize. A simple test, a modified chlorophenol red assay, was used for early identification of transgenic events expressing thepmigene. Transformation frequencies for both crops exceeded those obtained with thebarandpatgenes with selection on either Basta®or bialaphos. |
| Databáze: |
Supplemental Index |
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