| Autor: |
Hinman, Lee E., Sammak, Paul J. |
| Zdroj: |
Biotechniques; July 1998, Vol. 25 Issue: 1 p124-128, 5p |
| Abstrakt: |
Fura-2 is a popular real-time fluorescent indicator used to image intracellular, free calcium concentrations ([Ca2+]i) in living cells. One difficulty with fura-2-based digital imaging is how to best display the [Ca2+]iinformation that is acquired. Typically, only the [Ca2+]ias pseudocolor data are displayed, and the localization and intensity of the dye are not presented. This paper describes a technique that allows for the re-introduction of the intensity information. Recovery of the intensity information would de-emphasize regions with a poor signal-to-noise ratio and would more accurately report variations of sample morphology. A second advantage of this technique is that it requires no additional hardware since it is completely software based and can be run on all the major operating systems. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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