| Autor: |
Stimpson, Donald I., Cooley, Patrick W., Knepper, Sheila M., Wallace, David B. |
| Zdroj: |
Biotechniques; November 1998, Vol. 25 Issue: 5 p886-890, 5p |
| Abstrakt: |
Here, we present a low-cost method to produce compact arrays using microporous materials and reagent jetting. Oligonucleotides are immobilized on membrane sheets as a series of lines. The membrane sheet is then rolled and bound, and the spiral structure is cut like a “jelly roll” to produce identical arrays. The spiral arrays behave much like larger formats using membranes, and hybridization detection can be accomplished using standard signal-generation mechanisms. The method is particularly useful for producing identical arrays from pre-synthesized oligonucleotides. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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