| Autor: |
Marvik, Ole J., Isaksen, Morten L., Roza, Len, Lindqvist, Bjørn H. |
| Zdroj: |
Biotechniques; November 1997, Vol. 23 Issue: 5 p892-896, 5p |
| Abstrakt: |
We describe a simple detection system for DNA based on antibody detection of UV-induced photoproducts. It includes a convenient and inexpensive labeling procedure, which is completed in 5–10 min. The only equipment required is a UV source such as an ordinary transilluminator or a DNA crosslinker. Using a monoclonal antibody specific for thymine dimers, coupled to horseradish peroxidase, we are able to detect subpicogram amounts of UV-irradiated DNA directly, and approximately 10 pg homologues DNA indirectly by hybridization with an irradiated probe. |
| Databáze: |
Supplemental Index |
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