Isolation of High-Molecular-Length DNA from Human Skin

Autor: Bennett, P.V., Gange, R.W., Hacham, H., Hejmadi, V.S., Moran, M., Ray, S., Sutherland, B.M.
Zdroj: Biotechniques; September 1996, Vol. 21 Issue: 3 p458-463, 6p
Abstrakt: An agarose plug method for isolating high-molecular-length DNA from mammalian tissues has been developed, including from those that are difficult, such as skin. It gives high yields of DNA that contain a minimum of single-strand breaks and is readily digested by restriction and other nucleases. The method requires only simple equipment and is readily adaptable to field or clinical studies.
Databáze: Supplemental Index