A stable isotope technique for investigating lactate metabolism in humans

Autor: Neese, Richard A., Gertz, Edward W., Wisneski, Judith A., Gruenke, Larry D., Craig, J. Cymerman
Zdroj: Biomedical Mass Spectrometry; August 1983, Vol. 10 Issue: 8 p458-462, 5p
Abstrakt: A stable isotope tracer method has been developed for studying lactate metabolism in humans. The method uses lactic acid triply labeled with 13C as the tracer. The stable isotope is infused to attain a level of approximately 1.5% of that of the circulating unlabeled lactate. Following the isolation of lactic acid from the blood, the percentage of triply labeled (13C)lactate is measured using gas chromatography mass spectrometry. We compared this method with tracer methodology using [14C]lactate and found comparable results.
Databáze: Supplemental Index