| Autor: |
Hunt, Donald F., Buko, Alexander M., Ballard, John M., Shabanowitz, Jeffrey, Giordani, Anne B. |
| Zdroj: |
Biomedical Mass Spectrometry; September 1981, Vol. 8 Issue: 9 p397-408, 12p |
| Abstrakt: |
A new approach to the direct sequencing of oligopeptides in complex mixtures is described. Mixtures of [2H0]/[2H3]-N-acetylated and N, O-permethylated peptides are analyzed by collision activated dissociation on a triple quadrupole mass spectrometer using isobutane chemical ionization. Analysis of the collision activated dissociation spectra enables peptide sequences to be deduced. Use of electron capture negative chemical ionization for the sequence analysis of neuropeptides at the picomole level is also described. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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