| Autor: |
Ervik, Magnar, Hoffmann, Kurt-Jürgen, Kylberg-Hanssen, Kerstin |
| Zdroj: |
Biomedical Mass Spectrometry; July 1981, Vol. 8 Issue: 7 p322-326, 5p |
| Abstrakt: |
A highly sensitive and specific quantitative assay for metoprolol and two of its metabolites, containing an unchanged 2-hydroxy-4-isopropylaminopropoxy sidechain, has been developed. The compounds are isolated from the alkalized sample (plasma or urine) by extraction with dichloromethane, and converted to trifluoroacetyl derivatives by reaction with methyl-bis-(trifluoroacetamide). The reaction mixture is gas chromatographed on an OV-17 column and each substance is assayed by electron impact mass spectrometry using selected ion monitoring, and quantified by comparing the intensity of fragment ion m/z266 with the intensities of corresponding fragment ions from the deuterated internal standards (m/z270 and 271). It is possible to determine concentrations as low as 1 nmol I-1(0.3 ng ml-1) in 1 ml of sample with a relative standard deviation of less than 10%. |
| Databáze: |
Supplemental Index |
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