| Autor: |
Reiss, E., Patterson, D. G., Yert, L. W., Holler, J. S., Ibrahim, B. K. |
| Zdroj: |
Biomedical Mass Spectrometry; June 1981, Vol. 8 Issue: 6 p252-255, 4p |
| Abstrakt: |
Mannans of Candida albicansserotypes A and B and of Torulopsis glabratawere subjected to methylation analysis and the resulting monosaccharides were converted to the peracetylaldonitrile derivatives and subsequently analyzed by gas chromatography mass spectrometry. Serotype A differed from B in having more 1?2 linked mannosyl residues (46.2 vs 37.1 mol%) indicating longer oligomannosyl sidechains; mannosyl branch points linked through C-1, C-3 and C-6 were detected for the first time in C. albicansmannans and were more abundant in serotype B (9.2 vs 5.3 mol%). T. glabratamannan differed from that of C. albicansin having virtually no (1.6 mol%) unsubsituted sugars in the linear backbone and less 1?2 linked mannosyl residues (33.1 mol%) interpreted as shorter oligomannosyl sidechains. Model building of a repeating unit for the outer chain region of these mannase was prevented by the large amount of nonreducing terminal mannosyl residues, 23.0 mol% for C. albicansA, that probably arise from the inner core region. These mannans were exposed to exo-a-mannanase and the percentage of digestion, measured as reducing sugar released, was 33.5% for C. albicans B; 27.4% for T. glabrata, and C. albicans Awas refractroy (5.4% digested). |
| Databáze: |
Supplemental Index |
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