| Autor: |
Dejongh, D. C., Faus, G., Nayar, M. S. B., Boileau, G., Brakier-Gingras, L. |
| Zdroj: |
Biomedical Mass Spectrometry; August 1976, Vol. 3 Issue: 4 p191-195, 5p |
| Abstrakt: |
The terminal amino group of amino acids and peptides is blocked as the N-succinyl derivative by reaction with succinic anhydride. The product is then converted to the N,O-permethyl derivative in order to increase its volatility for use in mass spectrometry. The permethylated N-succinyl derivative retains the advantages of the permethylated N-acetyl derivative in regard to ease of preparation on a small scale, volatility and the presence of characteristic fragmentation patterns in their mass spectra. However, peaks in the high mass region are more abundant due to loss of CH3O· from the N-succinyl carbomethoxyl group as well as from the C-terminal carbomethoxyl group. Ions characteristic of the sequence and of individual amino acids are observed, and molecular weight can be determined from the relatively abundant ion at [M ? CH3O]+and from the weak molecular ion. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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