Surface arrays of energy absorbing polymers enabling covalent attachment of biomolecules for subsequent laser-induced uncoupling/desorption

Autor:	Voivodov, Kamen I, Ching, Jesus, Hutchens, T.William
Zdroj:	Tetrahedron Letters; January 1996, Vol. 37 Issue: 32 p5669-5672, 4p
Abstrakt:	Synthetic polymers with desirable film-forming characteristics have been chemically modified to incorporate, covalently, UV energy-absorbing molecules (EAM) of a type used for matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry. The polymer-bound EAM were activated to covalently bind macromolecules through the carboxyl group of the bound EAM. The EAM was shown to act as a photolabile macromolecular tether. Biomolecules covalently bound to surface arrays of EAM-polymer were uncoupled and desorbed/ionized with single pulses of laser irradiation.
Databáze:	Supplemental Index
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