| Autor: |
Grimsley, Gerald R., Trevino, Saul R., Thurlkill, Richard L., Scholtz, J. Martin |
| Zdroj: |
Current Protocols in Protein Science; February 2013, Vol. 71 Issue: 1 p28.4.1-28.4.14 |
| Abstrakt: |
This unit contains basic protocols for determining the conformational stability of a globular protein from either urea or thermal unfolding curves. Circular dichroism is the optical spectroscopic technique most commonly used to monitor protein unfolding. The protocols describe how to analyze data from an unfolding curve to obtain the thermodynamic parameters necessary to calculate conformational stability, and how to determine differences in stability between protein variants. Curr. Protoc. Protein Sci. 71:28.4.1‐28.4.14. © 2013 by John Wiley & Sons, Inc. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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