| Autor: |
Loakes, David, Brown, Daniel M., Salisbury, Stephen A., McDougall, Mark G., Neagu, Constantin, Nampalli, Satyam, Kumar, Shiv |
| Zdroj: |
Helvetica Chimica Acta; April 2003, Vol. 86 Issue: 4 p1193-1204, 12p |
| Abstrakt: |
A novel nucleoside analogue is described based on the pyridazine ring system. The nucleoside was successfully incorporated into DNA via both its phosphoramidite and 5'-triphosphate derivatives. Enzymatically, the analogue behaves essentially as thymidine: it is a good substrate for the DNA polymerases Taq and exonuclease-free Klenow fragment, leading to full-length products when present in either the primer or template strands. In hybridisation studies, the nucleoside displays ambiguous base-pairing properties, including universal base properties. |
| Databáze: |
Supplemental Index |
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