| Autor: |
Peroni, A., Mirabella, G., Sigala, S., Fornari, S., Palazzolo, F., Faccoli, S., Simeone, C., Pezzotti, G., Spano, P.F., Cunico, S. Cosciani |
| Zdroj: |
Urologia; January 2005, Vol. 72 Issue: 1 p10-13, 4p |
| Abstrakt: |
The aim of this work is to report a detailed study of the presence of α1AR subtypes in human urinary bladder areas involved in the micturition (i.e. detrusor, trigone and neck), investigating whether or not there are differences between sexes.Methods Urinary bladder specimens were obtained as discarded tissue after cystectomy for vesical carcinoma (17 men and 14 women). Molecular characterization of α1AR subtypes was done by semi-quantitative RT-PCR. The α1AR protein expression was studied by saturation binding curves using the α1AR antagonist 125I-HEAT and by western blot. Analysis of data was performed using the GraphPad PRISM 4 software.Results Results obtained indicated that α1AR proteins were detectable in each bladder area. In both sexes, the detrusor and the neck expressed similar levels of α1ARs: respectively, detrusor: 14.6 ± 1.2 in men and 13.1 ± 1.1 fmol/mg prot in women; neck: 16.9±3.2 in men and 17.5 ± 4.1 fmol/mg prot in women. In the trigone, significantly higher α1ARs were found in women compared to men (20.6 ± 1.1 vs 11.7 ± 0.7 fmol/mg prot). Subtype analysis indicated that in women, each area was endowed with mRNA and protein encoding for each α1AR subtype. The men detrusor expressed α1Aand α1DARs, while in the trigone and the neck, each subtype was present.Conclusions A selective distribution of α1ARs did exist between sexes and between the different areas involved in micturition, supporting evidence which indicates a role of different α1AR subtypes in the pathophysiology of the lower urinary tract symptoms. |
| Databáze: |
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