| Autor: |
Liu, Ying, Yan, Shuying, Yang, Fuguang, Li, Dongliang, Tang, Jianian, Liu, Guoxuan, Lin, Shiwan, Niu, Sufang, Yang, Yali |
| Zdroj: |
IOP Conference Series: Materials Science and Engineering; December 2017, Vol. 275 Issue: 1 p012011-012011, 1p |
| Abstrakt: |
An high-frequency protocol for induction of callus from anther explants of Ricinus communis was described. When anther explants of R. communis was cultured directly onto medium containing 6-benzylaminopurine (BA) induced formation of only poor quality callus that had a low induction frequency of anther callus (10.67%). However, treating the anther explants with high concentrations (7.5-120 mg/L) of BA solution for short time periods (5-80 min) helped to improve the induction frequency and enhance the quality of the callus formation significantly. The best callus induction (41.25%) was observed when anther explants were treated with 15 mg/L BA solution for 10 min before being inoculated onto hormone-free Murashige and Skoog (MS) medium for 30 days. In order to further optimize the culture system, after treated with 15 mg/L BA for 10 min, anther explants were inoculated on the hormone-free MS medium contained concentrations of sodium nitroprusside (SNP). The results showed that SNP significantly promoted the response of callus induction, especially when 8 mg/L SNP was applied, the the highest percentage of callus induction (60.37%) were gained. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
