| Autor: |
Lu, Cuiling, Zhang, Yaoyao, Zheng, Xiaoying, Song, Xueling, Yang, Rui, Yan, Jie, Feng, Huailiang, Qiao, Jie |
| Zdroj: |
SCIENCE CHINA Life Sciences; June 2018, Vol. 61 Issue: 6 p633-643, 11p |
| Abstrakt: |
In vitromaturation (IVM), the maturation in culture of immature oocytes, has been used in clinic for more than 20 years. Although IVM has the specific advantages of low cost and minor side effects over controlled ovarian stimulation, the prevalence of IVM is less than 1% of routine in vitrofertilization and embryo transfer techniques in many reproductive centers. In this review, we searched the MEDLINE database for all full texts and/or abstract articles published in English with content related to oocyte IVM mainly between 2000 and 2016. Many different aspects of the IVM method may influence oocyte potential, including priming, gonadotrophin, growth factors, and culture times. The culture conditions of IVM result in alterations in the oocyte or cumulus cell transcriptome that are not observed under in vivo culture conditions. Additionally, epigenetic modifications, such as DNA methylation or acetylation, are also different between in vitroand in vivocultured oocytes. In sum, current IVM technique is still not popular and requires more systematic and intensive research to improve its effects and applications. This review will help point our problems, supply evidence or clues for future improving IVM technique, thus assist patients for fertility treatment or preservation as an additional option. |
| Databáze: |
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