| Abstrakt: |
Efficient and reliable approaches for distinguishing barley cultivars are urgently needed for the malting, brewing, and feed grain industries. The potential of microsatellites, also known as simple sequence repeats (SSRs), to uniquely identify barley cultivars was evaluated on a sample of closely related, visually indistinguishable, white aleurone six-rowed malting and feed (general purpose) barley cultivars grown in Canada. A total of 38 SSRs were tested against the DNA extracted from leaf tissue of 11 barley cultivars. Seventeen SSRs showed allelic polymorphisms among the 11 cultivars, providing a total of 40 different alleles. By combining data for five SSR loci, all 11 cultivars could be distinguished. DNA extracted from single seeds gave allelic SSR patterns similar to leaf DNA and could be used for a rapid system of cultivar identification. |
