| Autor: |
Karbalaie Niya, Mohammad Hadi, Keyvani, Hossein, Safarnezhad Tameshkel, Fahimeh, Salehi-Vaziri, Mostafa, Teaghinezhad-S, Sedigheh, Bokharaei Salim, Farah, Monavari, Seyed Hamid Reza, Javanmard, Davod |
| Zdroj: |
Translational Oncology; June 2018, Vol. 11 Issue: 3 p593-598, 6p |
| Abstrakt: |
Human papillomavirus (HPV)is a common viral infection worldwide associated with a variety of cancers. The integration of the HPVgenome in these patients causes chromosomal instability and triggers carcinogenesis. The aim of this study was to investigate the HPV-16genome physical status in four major cancers related to HPVinfection. Formalin-fixed paraffin-embedded blocks from our previous projects on head and neck, colorectal, penile, and cervical cancers were collected, and HPV-16–positive specimens were used for further analysis. The DNA extraction copy number of E2and E7genes was calculated by qualitative real-time PCR method. Serially diluted standards that were cloned in PUC57plasmid were used. Standard curve and melting curve analysis was used for quantification. Of the 672 specimens studied, 76 (11.3%) were HPV-16positive. We found that 35.6% (16/45) were integrated. Statistical analysis showed that there were significant correlations between integration of HPV-16and cervical cancer end-stage carcinogenesis (P< .0001), episomal form, and ASCUS lesions (P= .045). Significant correlation in penile cancer patients was seen between the episomal form and high-grade cancer stage (P= .037). Integration is a major factor in the carcinogenesis mechanism of HPVand has different prevalence in various cancers with a higher rate in progression except in penile cancer. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
