| Autor: |
LUO, Xiang-dong, LIU, Jian, ZHAO, Jun, DAI, Liang-fang, CHEN, Ya-ling, ZHANG, Ling, ZHANG, Fantao, HU, Biao-lin, XIE, Jian-kun |
| Zdroj: |
Journal of Integrative Agriculture; February 2018, Vol. 17 Issue: 2 p265-275, 11p |
| Abstrakt: |
Cold stress is a major problem in rice production. To rapidly identify genes for cold tolerance in Dongxiang wild rice (DWR, Oryza rufipogonGriff.), sequencing-based bulked segregant analysis of QTL-seq method was used to resequence the extremely resistant (R) and susceptible (S) bulks of a backcross inbred lines (BILs) population (derived from Oryza sativa×O. rufipogon) and their parents. Single nucleotide polymorphisms (SNP)-index graphs and corresponding ρ(SNP-index) graphs (at 99 and 95% confidence levels) for R- and S-bulks detected a total of 2 609 candidate SNPs, including 58 candidate cold-tolerance genes. Quantitative real-time PCR analysis revealed that 5 out of the 58 candidate genes had significant differences in expression between O. sativaand O. rufipogon.Structural variation and functional annotations of the 5 candidate genes were also analyzed, and allowed us to identify 2 insertion-deletion (InDel) markers (12-7 and 12-16) that were linked with candidate genes on chromosome 12 in DWR. These results are helpful for cloning and using cold tolerance genes from common wild rice in cultivated rice. |
| Databáze: |
Supplemental Index |
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