| Autor: |
Ginders, Maximilian, Leschnik, Michael, Künzel, Frank, Kampner, Doris, Mikula, Claudia, Steindl, Georg, Eichhorn, Inga, Feßler, Andrea, Schwarz, Stefan, Spergser, Joachim, Loncaric, Igor |
| Zdroj: |
Acta Veterinaria Scandinavica; December 2017, Vol. 59 Issue: 1 p1-5, 5p |
| Abstrakt: |
The aim of the present study was to investigate the genetic relatedness and the antimicrobial resistance profiles of a collection of Austrian Streptococcus pneumoniaeisolates from companion animals and horses. A total of 12 non-repetitive isolates presumptively identified as S. pneumoniaewere obtained during routinely diagnostic activities between March 2009 and January 2017. Isolates were confirmed as S. pneumoniaeby bile solubility and optochin susceptibility testing, matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) mass spectrometry and sequence analysis of a part recAand the 16S rRNA genes. Isolates were further characterized by pneumolysin polymerase chain reaction (PCR) and genotyped by multilocus sequence typing (MLST). Antimicrobial susceptibility testing was performed and resistance genes were detected by specific PCR assays. All isolates were serotyped. Four sequence types (ST) (ST36, ST3546, ST6934 and ST6937) and four serotypes (3, 19A, 19F and 23F) were detected. Two isolates from twelve displayed a multidrug-resistance pheno- and genotype. This study represents the first comprehensive investigation on characteristics of S. pneumoniaeisolates recovered from Austrian companion animals and horses. The obtained results indicate that common human sero- (23F) and sequence type (ST36) implicated in causing invasive pneumococcal disease (IPD) may circulate in dogs. Isolates obtained from other examined animals seem to be host-adapted. |
| Databáze: |
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