A simplified procedure for the determination of aflatoxin B1in cottonseed meals

Autor: Velasco, James
Zdroj: Journal of the American Oil Chemists' Society; February 1969, Vol. 46 Issue: 2 p105-107, 3p
Abstrakt: A simplified procedure for the detection of aflatoxin B1in cottonseed meals has been developed. The procedure substantially reduces the time and cost of aflatoxin analysis. A single chromatographic column of celite is used to concentrate and purify the aflatoxin fractions. A new solvent system of ether-methanol-water (96:3:1) improves separation of aflatoxin fractions on TLC plates. This improvement permits measurement of B1spots in one sweep of the plate with a densitometer using a 10–15 mm slit. The number of samples which can be screened for aflatoxin contamination on one TLC plate is doubled by re-use of the plate. The use of technical reagents and stainless steel beakers also helps reduce the time and cost of analysis. The procedure is sensitive to about 5 µg of B1per kg of meal.
Databáze: Supplemental Index
Externí odkaz: