| Autor: |
O’Connor, Charmian J., Petricevic, Susan F., Coddington, Jan M., Stanley, Roger A. |
| Zdroj: |
Journal of the American Oil Chemists' Society; April 1992, Vol. 69 Issue: 4 p295-300, 6p |
| Abstrakt: |
A novel nuclear magnetic resonance (NMR) assay has been developed to monitor lipase-catalyzed esterification reactions without the need to extract and purify the individual components. The technique measures ratios of ester:alcohol signals and has been shown to be both efficient and reproducible. The assay has proven useful as a quick screen for the effect of varying conditions on the extent of esterification in various biphasic solvent systems and can be applied to both saturated and unsaturated long-chain fatty acid and alcohol substrates. The NMR ratio technique has been used to quantitate the extent of reaction in theCandida cylindracealipase-catalyzed synthesis of oleyl palmitate, stearyl palmitate, oleyl oleate, oleylgamma-linolenate and oleyl linoleate. The identity of these products was confirmed by high-resolution mass spectrometry. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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