| Autor: |
Levy, M. G., Flowers, J. R., Poore, M. F., Mullen, J. E., Khoo, L. H., Pote, L. M., Paperna, I., Dzikowski, R., Litaker, R. W. |
| Zdroj: |
Journal of Aquatic Animal Health; December 2002, Vol. 14 Issue: 4 p235-246, 12p |
| Abstrakt: |
Trematodes belonging to the genus Bolbophorushave recently been reported as the cause of substantial morbidity and mortality in cultured channel catfish Ictalurus punctatusin Mississippi and Louisiana. Previous investigators identified only a single species, B. confusus. In this investigation, genetic techniques were used to identify all stages of the parasite in all of its hosts. The 18s rRNA genes from specimens collected in Mississippi were sequenced and compared; this analysis revealed that there are two distinct species, B. damnificus(previously identified as B. confusus) and another, undescribed species. (Phylogenetic analysis indicated that a third species, B. levantinus, is also closely related to the Mississippi species.) Species-specific polymerase chain reaction assays capable of identifying and differentiating between these two parasites were developed. Both species were found to infect the first intermediate host (the ram's horn snail Planorbella trivolvis) in commercial channel catfish ponds, but only B. damnificuswas recovered from the fish themselves. The new, unidentified Bolbophorusspecies was determined to be highly pathogenic to a number of fish species. The contribution of B. damnificusto disease in cultured channel catfish remains undetermined. Future investigations of these parasites must now take into account the presence of two distinct species. |
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