Communications: Isolation of Several MycobacteriumSpecies from Fish

Autor: Lansdell, W., Dixon, B., Smith, N., Benjamin, L.
Zdroj: Journal of Aquatic Animal Health; March 1993, Vol. 5 Issue: 1 p73-76, 4p
Abstrakt: Several species of marine fish caught in the wild and of freshwater ornamental fish were used in this study. Infected organs (liver, spleen, and kidney) were sampled for mycobacteria. Decontaminated tissue samples were plated onto selective media for mycobacterial recovery. After initial isolation, fluorescent and acid-fast staining techniques identified bacterial colonies to genus. Profiles of biochemical growth characteristics were used to further identify the isolates to species. Five species of Mycobacterium were identified: M. simiae, M. scrofulaceum, M. marinum, M. chelonae, and M. fortuitum. Of these, M. simiaeand M. scrofulaceumhave not been previously reported from fish. Tissue samples containing focal granulomatous lesions were prepared for electron microscopic examination.
Databáze: Supplemental Index