| Autor: |
Baig, Abdul M, Zohaib, R, Tariq, S, Ahmad, HR |
| Zdroj: |
Future Microbiology; February 2018, Vol. 13 Issue: 2 p195-207, 13p |
| Abstrakt: |
Aim:This study intended to trace the evolution of acid–base buffers and water homeostasis in eukaryotes. Acanthamoeba castellanii was selected as a model unicellular eukaryote for this purpose. Homologies of proteins involved in pH and water regulatory mechanisms at cellular levels were compared between humans and A. castellanii. Materials & methods:Amino acid sequence homology, structural homology, 3D modeling and docking prediction were done to show the extent of similarities between carbonic anhydrase 1 (CA1), aquaporin (AQP), band-3 protein and H+pump. Experimental assays were done with acetazolamide (AZM), brinzolamide and mannitol to observe their effects on the trophozoites of A. castellanii. Results: The human CA1, AQP, band-3 protein and H+-transport proteins revealed similar proteins in Acanthamoeba. Docking showed the binding of AZM on amoebal AQP-like proteins. Acanthamoebashowed transient shape changes and encystation at differential doses of brinzolamide, mannitol and AZM. Conclusion:Water and pH regulating adapter proteins in Acanthamoeba and humans show significant homology, these mechanisms evolved early in the primitive unicellular eukaryotes and have remained conserved in multicellular eukaryotes. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
