| Autor: |
Shasha, David, Karel, Dan, Angiuli, Olivia, Greenblatt, Adam, Ghebremichael, Musie, Yu, Xu, Porichis, Filippos, Walker, Bruce D. |
| Zdroj: |
Journal of Leukocyte Biology; December 2016, Vol. 100 Issue: 6 p1425-1433, 9p |
| Abstrakt: |
Superior inhibition of viral replication by CD8+T cells is associated with better retention of functional qualities ex‐vivo, and is enhanced by IL‐15. Mechanisms modulating HIV‐specific CD8+ T cell‐mediated viral inhibition are not well defined. To delineate features of effective control, we compared the ability of CD8+T cells from HIV ECs and CPs to inhibit HIV ex vivo. ECs showed superior inhibition compared to HAART‐treated or untreated CPs in a typical VIA in which CD8+ T cells are rested 3 d before use (P= 0.025). In contrast, comparable antiviral activity was observed in freshly thawed cells. Rested CD8+T cells underwent apoptosis with preferential loss of HIV‐specific cells. EC CD8+T cells showed greater capacity to sustain polyfunctionality ex vivo compared with those of CPs, and incubation of CD8+T cells with IL‐15 augmented inhibition. These results indicate that superior ex vivo inhibition of viral replication by CD8+T cells from ECs is associated with enhanced retention of functional qualities and that in vitro antiviral function is enhanced by IL‐15. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Plný text