| Autor: |
McGarrity, S. T., Hyers, T. M., Webster, R. O. |
| Zdroj: |
Journal of Leukocyte Biology; August 1988, Vol. 44 Issue: 2 p93-100, 8p |
| Abstrakt: |
The effect of platelets and their products on in vitro responses of human neutrophils to f‐met‐leu‐phe (fMLP) and phorbol myristate acetate (PMA) was studied. Platelets produced a concentration‐dependent inhibition of neutrophil superoxide anion (O2‐) generation with maximum inhibition of the response to fMLP approaching 42.2 ± 5.4% and that to PMA approaching 85.9 ± 3.4%. Supernates of thrombin‐activated platelets produced maximal inhibition of neutrophil O2‐generation in response to fMLP (66.3 ± 4.2%) at a ratio of 10 platelet equivalents (PEQ) to 1 neutrophil but failed to affect the cells' response to PMA. In contrast, lysate of sonicated platelets Inhibited neutrophil O2‐generation in response to both fMLP (59.6 ± 2.0%) and PMA (90.1 ± 1.3 %). Biochemical characterization of platelet lysate identified at least two stimulus‐specific inhibitory activities which differ in size, thermal stability and enzyme sensitivity. Platelet lysate also maximally inhibited neutrophil degranulation and chemotaxis at a ratio of 20 PEQ to 1 neutrophil. Our findings indicate that physiologically achievable levels of platelets or their products modulate the response of neutrophils in a stimulus‐specific manner and are thereby capable of potentially limiting tissue damage which accompanies neutrophil activation during inflammation. |
| Databáze: |
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