| Autor: |
Hofman, Paul, Le Negrate, Gaëlle, Mograbi, Baharia, Hofman, Véronique, Brest, Patrick, Alliana‐Schmid, Annie, Flatau, Gilles, Boquet, Patrice, Rossi, Bernard |
| Zdroj: |
Journal of Leukocyte Biology; October 2000, Vol. 68 Issue: 4 p522-528, 7p |
| Abstrakt: |
Recruitment of polymorphonuclear leukocytes (PMNL) is a hallmark of both urinary and digestive infections caused byEscherichia coli. Cytotoxic necrotizing factor 1 (CNF‐1) is a toxin produced by uropathogenic E. colistrains that mediates its effects via the activation of small GTP‐binding proteins. However, the role and the consequences of CNF‐1 on PMNL physiology remain largely unknown. In this study, we provide evidence that CNF‐1 dramatically affects the PMNL cytoskeleton architecture by inducing an increased content of F‐actin. Furthermore, we demonstrate that CNF‐1 increases functional features of PMNL, such as superoxide generation and adherence on epithelial T84 monolayers, but significantly decreases their phagocytic function. Our results suggest that CNF‐1 may behave as a virulence factor in urinary or digestive infection by stimulating PMNL cytotoxicity as a result of its enhancing effect on their adherence to epithelial cells as well as the production of radical oxygen products. Moreover, the decreased phagocytosis of PMNL induced by CNF‐1 likely facilitates growth of bacteria. In these conditions, CNF‐1 would intervene in the initiation and in the perpetuation of the inflammatory process. |
| Databáze: |
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