| Abstrakt: |
Background:Biochemical markers harvested from gingival crevicular fluid (GCF) may be useful to identify and predict periodontal disease progression and to monitor the response to treatment. Ctelopeptide pyridinoline cross‐links (ICTP), a host‐derived breakdown product specific for bone, and interleukin‐1β (IL‐1), a potent boneresorptive cytokine, have been associated with periodontal tissue destruction. The aim of this study was to examine the effect of nonsurgical periodontal therapy on GCF levels of ICTP and IL‐1. Methods:Twenty‐five chronic periodontitis subjects were monitored at 8 sites per subject at baseline prior to scaling and root planing and 1, 3, and 6 months after therapy. Four shallow (probing depths <4 mm) and 4 deep (probing depths ≥5 mm) sites were monitored for both marker levels and clinical parameters. GCF was collected for 30 seconds on paper strips, and levels of ICTP and IL‐1 were determined using radioimmunoassay (RIA) and enzyme‐linked immunosorbent assay (ELISA) techniques, respectively. Clinical measurements included probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP). Results:Deep sites exhibited significantly (P<0.001) higher ICTP and IL‐1 levels compared to shallow sites at all time intervals. ICTP demonstrated a stronger association to clinical parameters than IL‐1 including a modest correlation (r = 0.40, P<0.001) between ICTP and attachment loss. Significant improvements in PD, CAL, and BOP were observed at 1, 3, and 6 months in all sites (P<0.01). However, non‐surgical mechanical therapy did not significantly reduce ICTP and IL‐1 levels over the 6‐month period. Further examination of subjects based on smoking status revealed that ICTP levels were significantly reduced at 3 and 6 months and IL‐1 levels reduced at 3 months among non‐smokers only. Conclusions:A single episode of non‐surgical mechanical therapy did not significantly reduce biochemical markers associated with bone resorption in patients exhibiting chronic periodontitis. Future longitudinal studies are warranted to specifically evaluate the relationship between C‐telopeptide pyridinoline cross‐links and periodontal disease progression. J Periodontol 2001;72:1045‐1051. |
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