| Autor: |
Vincent, Jack W., Falkler, William A., Heath, James R. |
| Zdroj: |
Journal of Periodontology; February 1984, Vol. 55 Issue: 2 p93-97, 5p |
| Abstrakt: |
Recent studies havedemonstrated an association of Eubacteriumsp. with the subgingival microflora of patients with chronic periodontitis. One species, Eubacterium brachy, was evaluated to determine the possible mechanisms by which this microorganism may contribute to this disease. An extracellular antigen was identified in the culture supernatant which reacted with antibodies in human sera. This antigen was isolated by methanol precipitation and purified by gel filtration. The purified extracellular antigen was reacted in vitrowith 45CaCl2‐labeled fetal rat bone in a bone resorptive bioassay. This antigen was shown to have a molecular weight of 170,000, to share a line of identity with a sonicated preparation of E. brachywhole cells and to result in increased 45CaCl2release from fetal rat bones when cultures were exposed to the purified extracellular antigen at concentrations of 10 to 53 μg/ml. |
| Databáze: |
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