| Autor: |
Lacerda Pigosso, Laurine, Baeza, Lilian Cristiane, Vieira Tomazett, Mariana, Batista Rodrigues Faleiro, Mariana, Brianezi Dignani de Moura, Veridiana Maria, Melo Bailão, Alexandre, Borges, Clayton Luiz, Alves Parente Rocha, Juliana, Rocha Fernandes, Gabriel, Gauthier, Gregory M., Soares, Celia Maria de Almeida |
| Zdroj: |
Virulence; October 2017, Vol. 8 Issue: 7 p1417-1434, 18p |
| Abstrakt: |
ABSTRACTParacoccidoides brasiliensisand Paracoccidioides lutzii, the etiologic agents of paracoccidioidomycosis, cause disease in healthy and immunocompromised persons in Latin America. We developed a method for harvesting P. brasiliensisyeast cells from infected murine lung to facilitate in vivotranscriptional and proteomic profiling. P. brasiliensisharvested at 6 h post-infection were analyzed using RNAseq and LC-MSE. In vivoyeast cells had 594 differentially expressed transcripts and 350 differentially expressed proteins. Integration of transcriptional and proteomic data indicated that early in infection (6 h), P. brasiliensisyeast cells underwent a shift in metabolism from glycolysis to β-oxidation, upregulated detoxifying enzymes to defend against oxidative stress, and repressed cell wall biosynthesis. Bioinformatics and functional analyses also demonstrated that a serine proteinase was upregulated and secreted in vivo. To our knowledge this is the first study depicting transcriptional and proteomic data of P. brasiliensisyeast cells upon 6 h post-infection of mouse lung. |
| Databáze: |
Supplemental Index |
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