| Autor: |
Inoue, Kimiko, Ogonuki, Narumi, Yamamoto, Yoshie, Noguchi, Yoko, Takeiri, Shuji, Nakata, Kumiko, Miki, Hiromi, Kurome, Mayuko, Nagashima, Hiroshi, Ogura, Atsuo |
| Zdroj: |
Cloning and Stem Cells (formerly Cloning: Science and Policy); December 01, 2002, Vol. 4 Issue: 4 p311-317, 7p |
| Abstrakt: |
Cloned rabbit embryos are characterized by their extremely poor postimplantation development, despite their high survivability until the blastocyst stage in vitro. This study examined whether the developmental failure of cloned rabbit embryos in vivo can be overcome by technical improvements to the activation protocol. Freshly collected cumulus cells were transferred into enucleated oocytes by intracytoplasmic injection. One to two hours later, the oocytes were activated by electroporation with Ca2+ or inositol 1,4,5-trisphosphate (IP3), which is known to induce repeated rises in intracellular Ca2+, as in normal fertilization. After transfer of embryos at the two- to four-cell stages, well-defined implantation sites with remnant fetal tissue were observed at term (day 28) only in the IP3-stimulation groups (0.9% and 5.8% per transferred embryo for single and triple stimulation groups, respectively). When some recipients in the same group were examined at days 16-20, a viable cloned fetus (day 19) with normal organogenesis was obtained. These findings clearly demonstrate that the oocyte activation protocol using IP3 enhances the postimplantation development of nuclear-transferred rabbit embryos. |
| Databáze: |
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