| Autor: |
Moreira, Ana S., Mil-Homens, Dalila, Sousa, Sílvia A., Coutinho, Carla P., Pinto-de-Oliveira, Ana, Ramos, Christian G., dos Santos, Sandra C., Fialho, Arsénio M., Leitão, Jorge H., Sá-Correia, Isabel |
| Zdroj: |
Virulence; August 2017, Vol. 8 Issue: 6 p782-796, 15p |
| Abstrakt: |
ABSTRACTDuring long-term lung infection in cystic fibrosis (CF) patients, Burkholderia cenocepaciafaces multiple selective pressures in this highly stressful and fluctuating environment. As a consequence, the initial infecting strain undergoes genetic changes that result in the diversification of genotypes and phenotypes. Whether this clonal expansion influences the pathogenic potential is unclear. The virulence potential of 39 sequential B. cenocepacia(recAlineage IIIA) isolates, corresponding to 3 different clones retrieved from 3 chronically infected CF patients was compared in this study using the non-mammalian infection hosts Galleria mellonellaand Caenorhabditis elegans. The isolates used in this retrospective study were picked randomly from selective agar plates as part of a CF Center routine, from the onset of infection until patients' death after 3.5 and 7.5 y or the more recent isolation date after 12.5 y of chronic infection. The infection models proved useful to assess virulence potential diversification, but for some isolates the relative values diverged in C. elegansand G. mellonella. Results also reinforce the concept of the occurrence of clonal diversification and co-existence of multiple phenotypes within the CF lungs, also with respect to pathogenicity. No clear trend of decrease (or increase) of the virulence potential throughout long-term infection was found but there is an apparent tendency for a clone/patient-dependent decrease of virulence when the G. mellonellamodel was used. The sole avirulent variant in both infection hosts was found to lack the small third replicon previously associated to virulence. Although possible, the in vivoloss of this nonessential megaplasmid was found to be a rare event (1 among a total of 64 isolates examined). |
| Databáze: |
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